Aldose Reductase, human recombinant | 7361

Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E.coli and purified by using conventional chromatography techniques.

7361 | Aldose Reductase human recombinant DataSheet

Biomolecule/Target: Aldose Reductase

Synonyms: Aldo-keto reductase family1, member B1, AKR1B1, ADR, ALDR1, ALR2, AR

Alternates names: Aldo-keto reductase family1, member B1, AKR1B1, ADR, ALDR1, ALR2, AR

Taglines: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds

NCBI Gene ID #: 231

NCBI Gene Symbol: AKR1B1

Gene Source: Human

Accession #: P15121

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is approximately 0.2-0.9 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH/min at 25°C. Specific activity was expressed in units/mg of protein.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 35.8 kDa (316 aa, 1-316 aa), confirmed by MALDI-TOF.

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF