Anti-WT1 Antibody | A1875

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

A1875 | Anti-WT1 Antibody DataSheet

Antibody Target: WT1

Target Alternative Name: WIT-2, GUD, AWT1, Wilms tumor 1, NPHS4, EWS-WT1, WT1, WAGR, WT33

Tag Line: Affinity purified Rabbit polyclonal to WT1

Category: Primary antibody

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Rat, Mouse, Human

Immunogen Sequence: Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1 (NP_001185480.1).

Accession #: P19544

Gene ID: 7490

Appearance: Liquid

Form: Liquid

Concentration:

Formulation: Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification: Affinity purification

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting

Positive Control:

Application And Usages: IHC 1:50 - 1:200, IF 1:50 - 1:200, WB 1:500 - 1:2000

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: True

Immunocytochemistry Verified: True

Immunofluorescence Verified: True

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: False

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False