Description
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
A1875 | Anti-WT1 Antibody DataSheet
Antibody Target: WT1
Target Alternative Name: WIT-2, GUD, AWT1, Wilms tumor 1, NPHS4, EWS-WT1, WT1, WAGR, WT33
Tag Line: Affinity purified Rabbit polyclonal to WT1
Category: Primary antibody
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Rat, Mouse, Human
Immunogen Sequence: Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1 (NP_001185480.1).
Accession #: P19544
Gene ID: 7490
Appearance: Liquid
Form: Liquid
Concentration:
Formulation: Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting
Positive Control:
Application And Usages: IHC 1:50 - 1:200, IF 1:50 - 1:200, WB 1:500 - 1:2000
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: True
Immunocytochemistry Verified: True
Immunofluorescence Verified: True
Immunoprecipitation Verified: False
FACS Verified: False
ELISA Verified: False
ChIP Verified: False
Dot Blot Verified: False
Flow Cytometry Verified: False
Additional Information
Size: |
100 uL |
Storage Condition: |
-20°C |
Shipping Condition: |
Gel Pack |
Shelf Life: |
12 months |