AOX1/2 | Plant alternative oxidase 1 and 2 | AS04 054

AOX1/2 | Plant alternative oxidase 1 and 2 | AS04 054 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms including Arabidopsis thaliana AOX1A. UniProt: Q39219, TAIR: At3g22370, AOX1B UniProt: O23913, TAIR:AT3G22360, AOX1C UniProt: O22048, TAIR: AT3G27620, and AOX2, UniProt: O22049, TAIR: AT5G64210, Solanum lycopersicum UniProt: Q7XBG9, Oryza sativa UniProt: Q7XT33, AOX1D, TAIR: AT1G32350

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunolocalization (IL), Western blot (WB)

Related Products: AS06 152 Anti-AOX1 | alternative oxidase from Chlamydomonas reinhardtii, rabbit antibodiesAS04 054PRE | AOX1/2 | plant alternative oxidase 1 and 2, pre-immune serumAS04 054S | AOX | AOX positive control/quantitation standardPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 750 (IL), 1 : 1000 for 10-20 µg of mitochondrial protein/lane detection (WB)

Molecular weight: 36-40 | 36-40 for Arabidopsis thaliana

Confirmed Reactivity: Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Brassica oleracea, Kandelia candel, Eriphorum vaginatum, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Oryza sativa, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Solanum lycopersicum, Solanum tuberosum, Symplocarpus renifolius, Physcomitrium patens, Tigriopus californicus, Triticum aestivum

Predicted Reactivity: Aegilops tauschii, Brachypodium distachyon, Capsella rubella, Citrus sinensis, Citrus clementina, Corylus heterophylla, Crocus sativus, Cucumis sativus, Daucus carota, Glycine max, Hypericum perforatum, Lotus japonicus, Malus x domestica, Medicago truncatula, Medicago sativa, Naegleria gruberi (amoeba), Nelumbo nucifera, Nicotiana benthamiana, Oryza brachyantha, Populus tremula, Picea sitchensis, Pyrus communis, Saccharum officinarum, Sauromatum venosum, Sorghum bicolor, Selaginella moellendorffii, Tetrahymena thermophila, Zea mays, Vigna radiata, Vigna unguiculata, Vitis viniferaSpecies of your interest not listed? Contact us

Not reactive in: Candidia albicans, Chlamydomonas reinhardtii (use an antibody to algal AOX1, AS06 152)

Additional Information: According to Konert et al. (2015) AOX antibody is recognizing AOX1A and AOX1D.This product can be sold containing ProClin if requested.

Background: Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G22370, AT5G64210

Category: Mitochondria | Respiration

Research Area: Plant Biology