APRIL Antibody [Sacha-1] | XA-1014

APRIL Antibody [Sacha-1] | XA-1014 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Human, Mouse

Homology: N/A

Immunogen: APRIL (monoclonal Sache-1) antibody was raised against recombinant human APRIL (aa. 105-250) .

Research Area: Chemokines & Cytokines, Cancer

Tested Application: ICC, Flow

Application: APRIL antibody can be used to recognize human and mouse APRIL by Flow Cytometry (excellent for mouse APRIL, also stains human APRIL) , and Immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5 μg/mL Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mAPRIL (thick line) . Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Sacha-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: APRIL (monoclonal Sache-1) antibody was purified to ≥95% in SDS-PAGE.

Clonality: Monoclonal

Clone: Sacha-1

Isotype: IgG2a

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS containing 0.02% sodium azide

Concentration: N/A

Storage Condition: APRIL antibody can be stored at 4˚C, stable for one year.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: N/A