APRIL Antibody [Sacha-2] | XA-1025

APRIL Antibody [Sacha-2] | XA-1025 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Human, Mouse

Homology: N/A

Immunogen: APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250) .

Research Area: Chemokines & Cytokines, Cancer

Tested Application: E, ICC, Flow

Application: APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture) , Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μm sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μg/mL for 1hr after blocking in PBS/1%BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T  cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line) . Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% sodium azide) containing 1 μg/mL of Sacha-2 antibody.  After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: APRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE.

Clonality: Monoclonal

Clone: Sacha-2

Isotype: IgG2a

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS containing 0.02% sodium azide

Concentration: N/A

Storage Condition: APRIL antibody can be stored at 4˚C, stable for one year.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: N/A