Ascorbate Oxidase | P1569

Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70 kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.This enzyme is involved in a redox system involving ascorbic acid. The enzyme is highly specific for L-ascorbic acid and a few analogs. The pH optimum for enzymatic activity is in the range of pH 5.5 to 7.0. The activity at pH 4.5 and 8.3 is approximately 50% maximal activity.

P1569 | Ascorbate Oxidase DataSheet

Biomolecule/Target :

Synonyms: L-Ascorbate, oxygen oxidoreductase, Ascorbase

Alternates names: L-Ascorbate, oxygen oxidoreductase, Ascorbase

Taglines: Converts ascorbic acid to dehydroascorbic acid

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Cucurbita sp.

Accession #:

Recombinant: FALSE

Source: Cucurbita sp.

Purity by SDS-PAGE:

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): 1,000 to 3,000 units per mg protein

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit will oxidize 1.0 umole of L-ascorbate to dehydroascorbate per minute at pH 5.6 at 25 °C.

Molecular Weight: The enzyme exists as a dimer with a molecular weight of approximately 140 kDa. The monomer molecular weight is approximately 65 to 70 kDa. It is a multicopper oxidase containing approximately 8 copper atoms per molecule.

Concentration:

Appearance: Lyophilized

Physical form description: Lyophilized powder containing buffers and sucrose as stabilizer.

Reconstitution Instructions: The enzyme is reconstituted in 4 mM sodium phosphate buffer, pH 5.6, with 0.05% (w/v) BSA.

Amino acid sequence: 552 amino acid

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans