Description
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70 kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.This enzyme is involved in a redox system involving ascorbic acid. The enzyme is highly specific for L-ascorbic acid and a few analogs. The pH optimum for enzymatic activity is in the range of pH 5.5 to 7.0. The activity at pH 4.5 and 8.3 is approximately 50% maximal activity.
P1569 | Ascorbate Oxidase DataSheet
Biomolecule/Target :
Synonyms: L-Ascorbate, oxygen oxidoreductase, Ascorbase
Alternates names: L-Ascorbate, oxygen oxidoreductase, Ascorbase
Taglines: Converts ascorbic acid to dehydroascorbic acid
NCBI Gene ID #:
NCBI Gene Symbol:
Gene Source: Cucurbita sp.
Accession #:
Recombinant: FALSE
Source: Cucurbita sp.
Purity by SDS-PAGE:
Assay:
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): 1,000 to 3,000 units per mg protein
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit will oxidize 1.0 umole of L-ascorbate to dehydroascorbate per minute at pH 5.6 at 25 °C.
Molecular Weight: The enzyme exists as a dimer with a molecular weight of approximately 140 kDa. The monomer molecular weight is approximately 65 to 70 kDa. It is a multicopper oxidase containing approximately 8 copper atoms per molecule.
Concentration:
Appearance: Lyophilized
Physical form description: Lyophilized powder containing buffers and sucrose as stabilizer.
Reconstitution Instructions: The enzyme is reconstituted in 4 mM sodium phosphate buffer, pH 5.6, with 0.05% (w/v) BSA.
Amino acid sequence: 552 amino acid
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
Additional Information
Storage Temperature: |
4.0°C |
Shipping: |
Gel Pack |
Shelf Life: |
6 months |