AtpB | Positive control/quantitation standard | AS03 030S

AtpB | Positive control/quantitation standard | AS03 030S | Gentaur UK, US & Europe Distribution

Immunogen: N/A

Host: N/A

Conjugation: N/A

Clonality: N/A

Isotype: N/A

Purity: N/A

Format: Lyophilized, in glycerol.

Tested Application: Western blot (WB)

Related Products: collection of other protein standardsAS03 030 | anti-ATP synthase subunit beta global chicken antibodyAS05 085 | anti-ATP synthase subunit beta global rabbit antibody collection of other global antibodiesPlant and algal protein extraction buffer

Recommended Dilutions: Standard curve: 3 loads are recommended (0.5, 2 and 4µl) .For most applications a sample load of 0.2µg of chlorophyll will give a AtpB signal in this range.Positive control: load per well: a 2µl load is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Molecular weight: in most gel systems AtpB migrates around 50-54 kDa

Confirmed Reactivity: N/A

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: Concentration: after adding 90 µl of dest. water final concentration of the standard is 0.27 pmol/µl.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Background: ATP synthase is the universal enzyme that stnthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.

Reconstitution: For reconstitution add 90 µl of milliQ water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Protein standards-quantitation

Research Area: Plant Biology