AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken antibodies) | AS03 030

AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken antibodies) | AS03 030 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3

Host: Chicken

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format: Liquid at 23, 3 µg/µl

Tested Application: Immunolocalization (IL), Immunogold (IG), Western blot (WB)

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Recommended Dilutions: 1 : 500 for localization of native enzyme by immunogold (IL), 1 : 5 000-1 : 8 000 (WB)

Molecular weight: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Confirmed Reactivity: Arabidopsis thaliana, Hordeum vulgare, Phaeodactylum tricornutum, Spartina alterniflora, Spinacia oleracea, Synechocystis PCC 6803, Synechococcus PCC 7942, beef muscle, Rat liver

Predicted Reactivity: Acinetobacter baumannii, Chlamydomonas reinhardtii, , Clostridium sp., Cyanobacteria, Glycine max, Nannochloropsis gaditana, Oryza sativa, marine Diatoms, Vitis vinifera, Yersinia sp. Species of your interest not listed? Contact us

Not reactive in: Archeal V-type ATP synthase

Additional Information: Results of immunogold studies using anti-AtpB antibody are published in Andersson et al. (2009) .

Background: ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.

Reconstitution: N/A

Storage: Store at 2-8°C.; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G08670, ATCG00480

Category: Global Antibodies

Research Area: Plant Biology