AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) (10 µl) | AS05 085-10

AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) (10 µl) | AS05 085-10 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1 At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)

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Recommended Dilutions: 1 : 100 (IF), 1 : 5000 (BN-PAGE), 1 : 2000-1 : 5 000 (WB)

Molecular weight: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Confirmed Reactivity: Agostis stolonifera cv. 'Penncross', Arabidopsis thaliana, Bacillus cereus, Chlamydomonas reinhardtii, Hordeum vulgare, Glycine max, Lycopersicum esulentum, Oryza sp. (roots, leafs, pollen), Nicotiana bentamiana, Nicotiana tabacum, Populus sp., Spinacia oleracea, Zea mays. Animal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria

Predicted Reactivity: Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp., Diatoms, Cyanobacteria, E.coli K-12, Vitis vinifera, Yersinia sp. Species of your interest not listed? Contact us

Not reactive in: Archeal V-type ATP synthase

Additional Information: Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011) .This antibody can be used as a loading control for bacteria, Bacillus cereus.

Background: ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.

Reconstitution: For reconstitution add 10 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G08670, ATCG00480

Category: Global Antibodies

Research Area: Plant Biology, Photosynthesis