AtpH | ATP synthase subunit c (chloroplastic) | AS05 071

AtpH | ATP synthase subunit c (chloroplastic) | AS05 071 | Gentaur UK, US & Europe Distribution

Immunogen: Purified c subunit from Spinacia oleracea UniProt: P69447.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

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Recommended Dilutions: 1 : 1000-1 : 10 000 (WB)

Molecular weight: 8 kDa (for Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus

Predicted Reactivity: Algae, Cannabis sativa, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus alba, Pinus thunbergii, Zea mays, Vitis vinifera Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e, g, 5 or 6 subunits) may occur

Background: F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: ATCG00140

Category: ATP synthase

Research Area: Plant Biology