451 Recombinant Proteins and Cell culture
AtpH | ATP synthase subunit c (chloroplastic) | AS05 071
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- 451-AS05 071
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Description
AtpH | ATP synthase subunit c (chloroplastic) | AS05 071 | Gentaur UK, US & Europe Distribution
Immunogen: Purified c subunit from Spinacia oleracea UniProt: P69447.
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
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Recommended Dilutions: 1 : 1000-1 : 10 000 (WB)
Molecular weight: 8 kDa (for Arabidopsis thaliana)
Confirmed Reactivity: Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
Predicted Reactivity: Algae, Cannabis sativa, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus alba, Pinus thunbergii, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e, g, 5 or 6 subunits) may occur
Background: F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Reconstitution: For reconstitution add 200 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: ATCG00140
Category: ATP synthase
Research Area: Plant Biology