AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies) | AS05 085

AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies) | AS05 085 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)

Related Products: AS05 085-10 | Anti-AtpB rabbit antibody, smaller pack size of AS05 085, rabbitantibodiesAS05 085PRE | AtpB | beta subunit of ATP synthase, pre-immune serumAS03 030 | Anti-AtpB hen antibody (developed to exactly the same peptide as rabbit antibody) AS03 030S | ATP synthase subunit beta protein standard for quantitation and positive controlAS08 304 | Anti-ATP synthase subunit alpha, rabbit antibodiesAS08 312 | Anti-ATP synthase subunit gamma rabbit antibodiesAS05 071 | Anti-ATP synthase subunit c rabbit antibodiesAS16 3976 | Anti-AtpB | Beta subunit of ATP synthase, mitochondrial, rabbit antibodiesPlant and algal protein extraction buffer

Recommended Dilutions: 1 : 100 (IF), 1 : 5000 (BN-PAGE), 1 : 2000-1 : 5 000 (WB)

Molecular weight: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Confirmed Reactivity: Arabidopsis thaliana, Bacillus cereus, Bryopsis corticulans, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Cyanidioschyzon merolae, Echinochloa crus/galli, Escherichia coli, Helicobacter pylori, Hordeum vulgare, Gladieria sulphuraria, Glycine max, Lycopersicum esulentum, Moniliophthora perniciosa, Nannochloropsis salina, Neochloris oleoabundans (chlorophyta), Nicotiana bentamiana, Nicotiana tabacum, Oryza sp. (roots, leafs, pollen), Pheodactylum tricornutum CCAP 1055/1, Pisum sativum, Plasmodium berghei, Populus sp., Robinia pseudoacacia, Scaphoideus titanus, Selaginella martensii, Solanum lycopersicum, Spinacia oleracea, Tetrabaena socialis, Toxoplasma gondii, Zea maysAnimal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria

Predicted Reactivity: Acinetobacter baumannii, Algae, Brassica napus, Cannabis sativa, Clostridioides difficile, Cyanobacteria, E.coli K-12, Galdieria sulphuraria, Manihot esculenta, Nicotiana plumbaginifolia, Saccharomyces cerevisiae, Salmonella typhimurium, Trichodesmium erythraeum, Triticum aestivum, Vitis vinifera, Zosteria marina, Yrsinia sp.Species of your interest not listed? Contact us

Not reactive in: Archeal V-type ATP synthase

Additional Information: Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011) .This antibody can be used as a loading control for bacteria, Bacillus cereus.

Background: ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G08670, ATCG00480

Category: Global Antibodies

Research Area: Plant Biology, Photosynthesis