Beclin 1 Antibody | 3663

Beclin is a Bcl-2 binding protein. Its structure includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal (NES). Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since over expression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects.

3663 | Beclin 1 Antibody DataSheet

Antibody Target: Beclin 1

Target Alternative Name: BECN1 , GT197 , Beclin-1 , beclin1 , ATG6 , VPS30

Tag Line:

Category: Beclin

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human, mouse

Immunogen Sequence: Synthetic peptide corresponding to residues around amino acid 25 of mouse Beclin 1

Accession #: O88597

Gene ID: 56208

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.2 mg/ml

Formulation: 30 µg (0.2 mg/ml) protein A purified rabbit anti-Beclin 1 polyclonal antibody in phosphate-buffered saline (PBS) containing 1% BSA, 50% glycerol, and 0.02% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control:

Application And Usages: Western blotting (0.5-2 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes ~53 kDa beclin 1 in Jurkat cell lysate. A weak band of ~110 kDa (possibly dimer) can also be observed in Jurkat cells. In mouse intestine tissue lysate, the antibody recognizes a ~100 kDa band (possibly dimer). Reactivity to other species has not been tested.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified: Not Verified

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified