Description
Beclin is a Bcl-2 binding protein. Its structure includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal (NES). Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since over expression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects.
3663 | Beclin 1 Antibody DataSheet
Antibody Target: Beclin 1
Target Alternative Name: BECN1 , GT197 , Beclin-1 , beclin1 , ATG6 , VPS30
Tag Line:
Category: Beclin
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human, mouse
Immunogen Sequence: Synthetic peptide corresponding to residues around amino acid 25 of mouse Beclin 1
Accession #: O88597
Gene ID: 56208
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.2 mg/ml
Formulation: 30 µg (0.2 mg/ml) protein A purified rabbit anti-Beclin 1 polyclonal antibody in phosphate-buffered saline (PBS) containing 1% BSA, 50% glycerol, and 0.02% thimerosal.
Purification: Affinity purified
Application: Western blot
Positive Control:
Application And Usages: Western blotting (0.5-2 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes ~53 kDa beclin 1 in Jurkat cell lysate. A weak band of ~110 kDa (possibly dimer) can also be observed in Jurkat cells. In mouse intestine tissue lysate, the antibody recognizes a ~100 kDa band (possibly dimer). Reactivity to other species has not been tested.
Country of Animal Origin: China
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified: Not Verified
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified
Additional Information
Size: |
30 ug |
Storage Condition: |
-20°C |
Shipping Condition: |
Gel Pack |
Shelf Life: |
12 months |