Benzo-Endonuclease | 9253

Benzo-Endonuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Benzo-Endonuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester bond. Benzo-Endonuclease reduces nucleic acid load and viscosity in cell lysates during purification to facilitate the filtration/ultrafiltration of solutions, reduces processing time, improves the separation of pellets and supernatants in centrifugations and increases the efficiency of chromatographic purification, resulting in significantly higher yield and purity of cell-derived particles such as viruses, AAV vectors or inclusion bodies.

9253 | Benzo-Endonuclease DataSheet

Biomolecule/Target :

Synonyms: Serratia marcescens Binuclease; unrestricted endonuclease

Alternates names: Serratia marcescens Binuclease; unrestricted endonuclease

Taglines: To digest all forms of DNA and RNA

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source:

Accession #:

Recombinant: True

Source: Genetically engineered Serratia marcescens endonuclease expressed in yeast cells

Purity by SDS-PAGE: ≥ 90% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level: Endotoxin free as determined by the LAL method

Activity (Specifications/test method): ≥ 1000 kU/mg protein

Biological activity: ≥ 20 kU/mg lyophilized powder

Results:

Binding Capacity:

Unit Definition: One unit of Benzo-Endouclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 min at pH 8.0 at 37 °C.

Molecular Weight: ~30 kDa

Concentration:

Appearance: Lyophilized solid

Physical form description:

Reconstitution Instructions: Reconstitute in 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl.

Amino acid sequence:

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans