EZSolution™ Benzo-Endonuclease | 9215

Benzo-Endonuclease is a genetically engineered non-specific endonuclease from Serratia marcescens gene. Functionally, this promiscuous nuclease digests all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Benzo-Endonuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester bond at all positions among a nucleic acid chain. The enzyme is produced and purified from eukaryotic yeast cells, without contamination of endotoxin from prokaryotic cells and is highly stable even under harsh industrial conditions. It has no detectable proteinase or esterase activity. Benzo-Endonuclease is an ideal tool enzyme in protein purification and DNA/RNA contamination from purified proteins

9215 | BenzAlt Binuclease Liquid DataSheet

Biomolecule/Target: N/A

Synonyms: Serratia marcescens binuclease; unrestricted endonuclease

Alternates names: Fatty acid-binding protein 1 liver, L-FABP, FABPL, FABP-1, FABP1, Z-protein.

Taglines: For digestion of DNA and RNA

NCBI Gene ID #: 2168

NCBI Gene Symbol: FABP1

Gene Source: N/A

Accession #: AAH22287

Recombinant: Produced and purified from eukaryotic yeast cells

Source: Serratia marcescens gene

Purity by SDS-PAGEs: 98%

Assay: SDS PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: None

Activity (Specifications/test method): 1.0 x 106 KU/mg

Biological activity: Test in process

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit (U) of Binuclease is defined as the amount of enzyme that causes a A260 of 1.0 in 30 min under the condition above, which means complete digestion of 37 g DNA

Molecular Weight: 19 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A