65

EnzyChrom™ NAD/NADH Assay Kit

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SKU:
65-E2ND-100-GEN
Availability:
In Stock
zł6,031.20

Description

EnzyChrom™ NAD/NADH Assay Kit

 

Application: For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.

Key Features:

  • Sensitive and accurate. Detection limit of 0.05 μM and linearity up to 10 μM NAD+/NADH in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method: OD565nm

Samples: Cell or tissue extracts

Species: All

Size: 100 tests

Detection Limit: 0.05 μM

Shelf Life: 6 months

More Details:

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD+/NADH concentration are very desirable.

Gentaur Genprice EnzyChrom™ NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.

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Additional Information

Usage:
Sensitive determination of NAD and NADH by colorimetric (565nm) method.
Procedure:
15 min
Size:
100 Tests
Detection limit:
0.05 µM.
Shelf life:
6 months.
Shipping:
on ice
Storage:
-20°C.
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1 Review

  • 4
    Efficient

    Posted by Amir on 30th Sep 2022

    La compañía ofrece una amplia gama de valiosos servicios y productos. Bien hecho, Gentauro. Buen trabajo.

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