EnzyChrom™ NAD/NADH Assay Kit

Application: For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.

Key Features:

• Sensitive and accurate. Detection limit of 0.05 μM and linearity up to 10 μM NAD+/NADH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method: OD565nm

Samples: Cell or tissue extracts

Species: All

Size: 100 tests

Detection Limit: 0.05 μM

Shelf Life: 6 months

More Details:

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD⁺/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD⁺/NADH concentration are very desirable.

Gentaur Genprice EnzyChrom™ NAD⁺/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD⁺/NADH concentration in the sample. This assay is highly specific for NAD⁺/NADH and with minimal interference (<1%) by NADP⁺/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.