EnzyChrom™ Phospholipd Assay Kit

Application: For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.

Key Features:

Sensitive. Use 20 μL samples. Linear detection range: colorimetric assay 3 - 200 μM, fluorimetric assay 0.6 - 20 μM phospholipid.

Method: OD570nm, or FL530/585nm

Samples: Biological

Species: All

Size: 100 tests

Detection Limit: OD, FL: 3, 0.6 μM

Shelf Life: 6 months

More Details: 

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. Gentaur Genprice method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples.

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H₂O₂ specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.