65

EnzyChrom™ Pyruvate Assay Kit

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SKU:
65-EPYR-100-GEN
Availability:
In Stock
NULL887.60

Description

EnzyChrom™ Pyruvate Assay Kit

 

Application: For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.

Key Features:

  • Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorimetric assays.
  • Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.
  • Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Method: OD570nm, or FL530/585nm

Samples: Biological

Species: All

Size: 100 tests

Detection Limit: OD, FL: 2, 0.2 μM

Shelf Life: 6 months

More Details:

Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.

Gentaur Genprice pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.

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Additional Information

Usage:
Quantitative determination of pyruvate by colorimetric (570nm) or fluorimetric (530nm/590nm) methods.
Procedure:
20 min
Size:
100 Tests
Detection limit:
fluorimetric assay 0.2 µM, colorimetric assay 2 µM.
Shelf life:
6 months.
Shipping:
on ice
Storage:
-20°C
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1 Review

  • 4
    its good

    Posted by Emmett on 30th Sep 2022

    He tenido una buena experiencia con Gentaur. Los productos y servicios que ofrecen son incomparables.

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