EnzyChrom™ Pyruvate Assay Kit
Application: For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
Key Features:
- Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorimetric assays.
- Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.
- Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Method: OD570nm, or FL530/585nm
Samples: Biological
Species: All
Size: 100 tests
Detection Limit: OD, FL: 2, 0.2 μM
Shelf Life: 6 months
More Details:
Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.
Gentaur Genprice pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.
