EnzyChrom™ Superoxide Dismutase Assay Kit

Application: For quantitative determination of superoxide dismutase (SOD) enzyme activity.

Key Features:

• Sensitive and accurate. Linear detection range of 0.05 - 3 U/mL SOD.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method: OD440nm

Samples: Blood, cell, tissue etc

Species: All

Size: 100 tests

Detection Limit: 0.05 U/mL

Shelf Life: 6 months

More Details:

Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O₂ and H₂O₂. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer.

Gentaur Genprice SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O₂^-) is provided by xanthine oxidase (XO) catalyzed reaction. O₂^- reacts with a WST-1 dye to form a colored product. SOD scavenges the O₂^- thus less O₂^- is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.