EnzyFluo™ NADP/NADPH Assay Kit

Application: For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

Key Features:

• Sensitive and accurate. Detection limit of 0.01 μM and linearity up to 1 μM NADP+/NADPH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method: FL530/585nm

Samples: Cell, tissue extracts etc

Species: All

Size: 100 tests

Detection Limit: 0.01 μM

Shelf Life: 6 months

More Details:

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP⁺/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP⁺/NADPH concentration are very desirable.

Gentaur Genprice EnzyFluo™ NADP⁺/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NADP⁺/NADPH concentration in the sample. This assay is highly specific for NADP⁺/NADPH and with minimal interference (<1%) by NAD⁺/NADH. Our assay is a convenient method to measure NADP⁺, NADPH and their ratio.