Description
Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs thro?gh phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.
3442 | Erk2 Antibody DataSheet
Antibody Target: Erk-2
Target Alternative Name: ERK , ERK-2 , MAPK2 , PRKM2, Extracellular signal-regulated kinase 2; ERK2; Defective in aggregation protein C; MAP kinase 2, Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; Extracellular signal-regulated kinase 2; ERK-2; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAP kinase isoform p42; p42-MAPK; ERT1.
Tag Line:
Category: Erk
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human, mouse, rat
Immunogen Sequence: Synthetic peptide from human Erk2
Accession #: P28482.3
Gene ID: 119554
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Purification: Affinity purified
Application: Western blot, Immunohistochemistry
Positive Control:
Application And Usages: Western blotting (0.5-4 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually.
Country of Animal Origin: China
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified:
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified
Additional Information
Size: |
100 ug |
Storage Condition: |
-20°C |
Shipping Condition: |
Gel Pack |
Shelf Life: |
12 months |