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EZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant | 7868
- SKU:
- 26-7868-GEN
- Availability:
- Usually shipped in 5 working days
Description
SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal His-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO Protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Ni chelating resin (Cat. # 6562-1, 10, 100, 500).
7868 | EZCut SUMO Protease 1 His-tagged Yeast Recombinant DataSheet
Biomolecule/Target: SUMO Protease 1
Synonyms: Ulp1 peptidase, SUMO Protease
Alternates names: Resistin-like , RELM, Cysteine-rich secreted protein FIZZ1, Parasite-induced macrophage novel gene 1 protein, Cysteine-rich secreted protein A12-, RELM-a
Taglines: >90% Pure, Active, Yeast Recombinant, His-Tagged SUMO Protease 1, to cleave the SUMO tag from recombinant proteins.
NCBI Gene ID #: 57262
NCBI Gene Symbol: RETNA
Gene Source: Yeast
Accession #: Q9EP95
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): The EZCut™ SUMO Protease 1 (His-tagged) has an activity of 1 X 106 units/mg.
Biological activity: Test in process
Results: 1 X 106 units/mg.
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of EZCut™ SUMO Protease 1 required to cleave >90% of 5 µg a control protein substrate (SUMO-GFP) in 1 h at 37°C.
Molecular Weight: 10.0 kDa
Concentration: 0.1 mg/ml (100 U/µl)
Appearance: Liquid
Physical form description: 0.1 mg/ml (100 U/µl) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 g/l. The solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A