GAPDH, mouse recombinant | P1099

Gapdh, also known as glyceraldehyde 3-phosphate dehydrogenase, is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, Gapdh has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. Recombinant mouse Gapdh, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1099 | GAPDH mouse recombinant DataSheet

Biomolecule/Target: GAPDH

Synonyms: G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2

Alternates names: Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP

Taglines: Involved in glycolysis

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Mouse

Accession #:

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

Biological activity: 5 x 10IU/mg.

Results: > 40 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

Molecular Weight: 6.5 kDa

Concentration: 0.25 mg/ml

Appearance: Liquid

Physical form description: In Phosphate buffer saline (pH 7.4)containing 20% glycerol 1mM DTT.

Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use. For long-term storage, it is recommend to add a carrier protein (e.g., 0.1% BSA). Prevent freeze/thaw cycles.

Amino acid sequence: N/A