26
GAPDH, mouse recombinant | P1099
- SKU:
- 26-P1099-GEN
- Availability:
- Usually shipped in 5 working days
Description
Gapdh, also known as glyceraldehyde 3-phosphate dehydrogenase, is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, Gapdh has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. Recombinant mouse Gapdh, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
P1099 | GAPDH mouse recombinant DataSheet
Biomolecule/Target: GAPDH
Synonyms: G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2
Alternates names: Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP
Taglines: Involved in glycolysis
NCBI Gene ID #:
NCBI Gene Symbol:
Gene Source: Mouse
Accession #:
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
Biological activity: 5 x 10IU/mg.
Results: > 40 units/mg
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
Molecular Weight: 6.5 kDa
Concentration: 0.25 mg/ml
Appearance: Liquid
Physical form description: In Phosphate buffer saline (pH 7.4)containing 20% glycerol 1mM DTT.
Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or 20°C for future use. For long-term storage, it is recommend to add a carrier protein (e.g., 0.1% BSA). Prevent freeze/thaw cycles.
Amino acid sequence: N/A