GDNF, murine recombinant | 7156

GDNF is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFR (1-4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

7156 | GDNF murine recombinant DataSheet

Biomolecule/Target: GDNF

Synonyms: Glial-Derived Neurotrophic Factor, ATF-1

Alternates names: Apolipoprotein E3, APOE, apolipoprotein, apolipoproteins

Taglines: Promotes dopamine uptake and survival and morphological differentiation of midbrain neurons

NCBI Gene ID #: 348

NCBI Gene Symbol: APOE

Gene Source: Human

Accession #: NM_000041

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): The ED50 was determined by the proliferation of rat C6 cells is 0.2 ng/ml, corresponding to a specific activity of 5 x 10^6 units/mg.

Biological activity: Test in process

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 34 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium citrate.

Reconstitution Instructions: Reconstitute in dHO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A