General 2-AG (2-Arachidonoylglycerol) ELISA Kit | ELK8163

General 2-AG (2-Arachidonoylglycerol) ELISA Kit | ELK8163 | Gentaur UK, US & Europe Distribution

Alternative Names: 2-AG

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.41 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 2-Arachidonoylglycerol (2-AG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 2-Arachidonoylglycerol (2-AG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 2-Arachidonoylglycerol (2-AG) in the samples is then determined by comparing the OD of the samples to the standard curve.