GPT/ALT1, human recombinant | 7805

GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

7805 | GPT/ALT1 human recombinant DataSheet

Biomolecule/Target: GPT/ALT1

Synonyms: Alanine aminotransferase 1, AAT1, ALT1, GPT1

Alternates names: Alanine aminotransferase 1, AAT1, ALT1, GPT1

Taglines: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate

NCBI Gene ID #: 26291

NCBI Gene Symbol: FGF-21

Gene Source: Human

Accession #: Q9NSA1

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 20 unit/mg; One unit will convert 1.0 µmole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37ºC.

Results: Specific activity is > 20 unit/mg

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37ºC.

Molecular Weight: 56.8 kDa (516 aa, 1-496 aa + His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol and 1 mM DTT.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS