26
GPT/ALT1, human recombinant | 7805
- SKU:
- 26-7805-GEN
- Availability:
- Usually shipped in 5 working days
Description
GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
7805 | GPT/ALT1 human recombinant DataSheet
Biomolecule/Target: GPT/ALT1
Synonyms: Alanine aminotransferase 1, AAT1, ALT1, GPT1
Alternates names: Alanine aminotransferase 1, AAT1, ALT1, GPT1
Taglines: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate
NCBI Gene ID #: 26291
NCBI Gene Symbol: FGF-21
Gene Source: Human
Accession #: Q9NSA1
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is > 20 unit/mg; One unit will convert 1.0 µmole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37ºC.
Results: Specific activity is > 20 unit/mg
Binding Capacity: N/A
Unit Definition: One unit will convert 1.0 µmole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37ºC.
Molecular Weight: 56.8 kDa (516 aa, 1-496 aa + His Tag)
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol and 1 mM DTT.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS