H3 | Histone H3 (rabbit antibody) (nuclear marker) | AS10 710

H3 | Histone H3 (rabbit antibody) (nuclear marker) | AS10 710 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030, At4g40040, At5g10980), H3.2 P59226 (At1g09200, At3g27360, At5g10390, At5g10400, At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: ChIp-qPCR (ChIp-qPCR), Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)

Related Products: AS11 1804 | Anti-RNA polymerase II subunit B1, rabbit antibodyAS16 3197 | Anti-H3 | Histone 3 core, monoclonal antibodyAS15 2855 | Anti-H3 | histone H3, chicken antibodyAS10 710-ALP | Anti-H3 | Histone H3, ALP-conjugated (40 µg) AS10 710-HRP | Anti-H3 | Histone H3, HRP-conjugated (40 µg) AS10 710PRE | H3 | histone H3, pre-immune serum (control for immunolocalization) collection of antibodies to DNA/RNA/cell cyclePlant and algal protein extraction buffer

Recommended Dilutions: 1 : 100-1 : 500 (ICC), 2 µl of antibody/500 µl solution (ChIp-qPCR), 1: 500 (IF), 1 : 5000 (WB)

Molecular weight: 15 | 17 kDa

Confirmed Reactivity: Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Phalaenopsis equestris, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays

Predicted Reactivity: Brachypodium distachyon, Brassica napus, Emiliania huxleyi, Nannochloropsis gaditana, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Ostreococcus sp., Sorghum bicolor, Vitis vinifera, Volvox sp. Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes

Background: Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G40030

Category: Nuclear marker

Research Area: Cell Biology