Description
HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to the epidermal growth factor receptor. HER2 is an oncogene and overexpression of unaltered HER2 coding sequences in NIH 3T3 cells results in cellular transformation and tumorigenesis (1). HER2 is amplified in about 30% of primary human breast malignancies and overexpression of HER2 is associated with the most aggressive tumors that show uncontrolled proliferation, resistance to apoptosis and increased motility (2).
Biomolecule/Target: N/A
Synonyms: HER, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
Alternates names: Insulin-like Growth Factor-Binding Protein 3, Growth-hormone-dependant binding protein
Taglines: Plays an important role in the pathogenesis and progression of aggressive types of cancer
NCBI Gene ID #: 3486
NCBI Gene Symbol: IBP3
Gene Source: N/A
Accession #: P17936
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): The specific activity of HER2 was determined to be 10 nmol /min/mg as per activity assay protocol.
Biological activity: The ED as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is <0.2 mg/ml, in the presence of 15 ng/ml of human IGF-II.
Results: <0.2 mg/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 28.8 kDa
Concentration: 0.1mg/ml
Appearance: Liquid
Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or 20°C for future use.
Amino acid sequence: AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS