HER2, Active | 7762

HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to the epidermal growth factor receptor. HER2 is an oncogene and overexpression of unaltered HER2 coding sequences in NIH 3T3 cells results in cellular transformation and tumorigenesis (1). HER2 is amplified in about 30% of primary human breast malignancies and overexpression of HER2 is associated with the most aggressive tumors that show uncontrolled proliferation, resistance to apoptosis and increased motility (2).

7762 | HER2 Active DataSheet

Biomolecule/Target: N/A

Synonyms: HER, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

Alternates names: Insulin-like Growth Factor-Binding Protein 3, Growth-hormone-dependant binding protein

Taglines: Plays an important role in the pathogenesis and progression of aggressive types of cancer

NCBI Gene ID #: 3486

NCBI Gene Symbol: IBP3

Gene Source: N/A

Accession #: P17936

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of HER2 was determined to be 10 nmol /min/mg as per activity assay protocol.

Biological activity: The ED as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is <0.2 mg/ml, in the presence of 15 ng/ml of human IGF-II.

Results: <0.2 mg/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 28.8 kDa

Concentration: 0.1mg/ml

Appearance: Liquid

Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS