Histidine Decarboxylase, M. morganii Recombinant | P1442

Histidine decarboxylase is the only enzyme that is known to generate histamine. Histamine is important for signal transduction, immune response, and gastric acid secretion.

P1442 | Histidine Decarboxylase, M. morganii Recombinant DataSheet

Biomolecule/Target :

Synonyms: HDC

Alternates names: HDC

Taglines: Catalyzes the biosynthesis of histamine from histidine.

NCBI Gene ID #: P05034.2

NCBI Gene Symbol: hdc

Gene Source: M. morganii

Accession #: P05034

Recombinant: True

Source: M. morganii

Purity by SDS-PAGE: >90%

Assay: SDS-PAGE.

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): This enzyme has a specific activity of ≥ 600 mU/mg based on its conversion of histidine to histamine, which can be detected calorimetrically at OD 450 nm using ’s Histamine Assay Kit (K506).

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit is the amount of enzyme that will convert 1.0 umole of histidine to histamine per minute at pH 8.0 and 37 ÌŠC.

Molecular Weight: 42.8 kDa with N-terminal 6x-His tag

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute the lyophilized protein in 50 mM Sodium Phosphate, 30% Glycerol and 5 mM BME at 0.5 mg/ml. Incubate the reconstituted protein at 25°C for 15 minutes.

Amino acid sequence: aa 1 – 378

Handling: Centrifuge the vial prior to opening.

Usage: