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Histidine Decarboxylase, M. morganii Recombinant | P1442
- SKU:
- 26-P1442-GEN
- Availability:
- Usually shipped in 5 working days
Description
Histidine decarboxylase is the only enzyme that is known to generate histamine. Histamine is important for signal transduction, immune response, and gastric acid secretion.
P1442 | Histidine Decarboxylase, M. morganii Recombinant DataSheet
Biomolecule/Target :
Synonyms: HDC
Alternates names: HDC
Taglines: Catalyzes the biosynthesis of histamine from histidine.
NCBI Gene ID #: P05034.2
NCBI Gene Symbol: hdc
Gene Source: M. morganii
Accession #: P05034
Recombinant: True
Source: M. morganii
Purity by SDS-PAGE: >90%
Assay: SDS-PAGE.
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): This enzyme has a specific activity of ≥ 600 mU/mg based on its conversion of histidine to histamine, which can be detected calorimetrically at OD 450 nm using ’s Histamine Assay Kit (K506).
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit is the amount of enzyme that will convert 1.0 umole of histidine to histamine per minute at pH 8.0 and 37 ÌŠC.
Molecular Weight: 42.8 kDa with N-terminal 6x-His tag
Concentration:
Appearance: Lyophilized powder
Physical form description: Lyophilized
Reconstitution Instructions: Reconstitute the lyophilized protein in 50 mM Sodium Phosphate, 30% Glycerol and 5 mM BME at 0.5 mg/ml. Incubate the reconstituted protein at 25°C for 15 minutes.
Amino acid sequence: aa 1 – 378
Handling: Centrifuge the vial prior to opening.
Usage:
Additional Information
Storage Temperature: |
Store at -20°C. Aliquot and store the reconstituted enzyme at -20°C and use within 2 months. Avoid repeated freeze-thaw cycles. |
Shipping: |
Gel Pack |
Shelf Life: |
12 months |