Human CellExp™ B7-1 / CD80, mouse recombinant | P1075

B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA- 4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-B-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

P1075 | Human CellExp B7-1 / CD80 mouse recombinant DataSheet

Biomolecule/Target: CD80

Synonyms: CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

Alternates names: Neuregulin-1, NRG1, GGF, HGL, HRGA, NDF, SMDF, HRG, ARIA, GGF2, HRG1

Taglines: Plays a role in induction of innate immune responses by activating NF-B-signaling pathway in macrophages

NCBI Gene ID #: 3084

NCBI Gene Symbol: NRG1

Gene Source: Mouse

Accession #: Q02297

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: N/A

Results: N/A

Binding Capacity: Immobilized Mouse B7-1, His Tag at 2g/Ml (100 L/well) can bind Mouse CTLA-4, Fc Tag with a linear range of 0.12-0.5 ng/mL.

Unit Definition: N/A

Molecular Weight: 7.4 kDa (65 aa, 177-237)

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: N/A

Amino acid sequence: N/A