Human CellExp™ CD58 / LFA-3, Fc Tag, human recombinant | P1150

CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) [3] on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.

P1150 | Human CellExp CD58 / LFA-3 Fc Tag human recombinant DataSheet

Biomolecule/Target: CD58

Synonyms: CD58, LFA3, Ag3

Alternates names: Growth hormone 1, Somatotropin, Pituitary growth hormone

Taglines: Ligand of T-lymphocyte CD2 glycoprotein

NCBI Gene ID #: 378781

NCBI Gene Symbol: SOMA

Gene Source: Human

Accession #: P08998

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: GH Chicken Recombinantis fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.

Results: N/A

Binding Capacity: Immobilized Human CD2, His Tag at 2 g/mL (100 L/well) can bind Human CD58, Fc Tag with a linear range of 10-156 ng/mL

Unit Definition: N/A

Molecular Weight: 22.255 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile HO or 0.4 % NaHCO adjusted to pH 8-9 to a concentration 100 µg/ml and sterile filtered. This solution can then be diluted into other aqueous buffers, preferably in the presence of carrier protein.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala