Human CellExp™ HMGB1 /HMG1, Mouse Recombinant | P1465

High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.

P1465 | Human CellExp™ HMGB1 /HMG1, Mouse Recombinant DataSheet

Biomolecule/Target :

Synonyms: HMGB1, HMG1, HMG3, SBP-1

Alternates names: HMGB1, HMG1, HMG3, SBP-1

Taglines: A DNA vaccine adjuvant and a target for cancer therapy.

NCBI Gene ID #: 15289

NCBI Gene Symbol: Hmgb1

Gene Source: Mouse

Accession #: P63158

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: >90%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level: Less than 1.0 EU per ug by the LAL method.

Activity (Specifications/test method):

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.8 kDa. The protein migrates as 33 kDa and 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Amino acid sequence: Met 1 - Glu 215

Handling: Centrifuge the vial prior to opening.

Usage: