Human CellExp™ Vitronectin (His Tag, Active), Mouse Recombinant | P1754

Human CellExp™ Vitronectin (His Tag, Active), Mouse Recombinant | P1754

Description: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interacts with glycosaminoglycans and proteoglycans, and functions as a regulator of diverse physiological processes such as blood coagulation and cell attachment and spreading. The molecule can be recognized by certain integrin family members and serves as a cell-to-substrate adhesion molecule. Vitronectin also inhibits the membrane-damaging effect of the complement pathway. Due to its many diverse functions, vitronectin is implicated in coronary atherosclerosis, hemostasis, and tumor malignancy.

Biomolecule/Target:

Synonyms: S-Protein; Serum-spreadking factor; Vn; Vtn; AI256434 protein

Alternates names: S-Protein; Serum-spreadking factor; Vn; Vtn; AI256434 protein

Taglines: Promotes cell adhesion and spreading

NCBI Gene ID #: 22370

NCBI Gene Symbol: Murine

Accession #: P29788

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: > 85% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level: < 1.0 EU per µg as determined by the LAL method.

Activity (Specifications/test method): Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse vitronectin coated plates (10 μg/mL, 100 μL/well), > 60% cells will adhere specifically after 30 minutes at 37 °C

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 75 to 85 kDa (observed due to glycosylation)

Concentration:

Appearance: Lyophilized protein

Physical form description: Lyophilized powder

Reconstitution Instructions:

Amino acid sequence: Met 1 to Lys 478 (predicted N-terminus is Asp 20) with C-terminal polyhistidine tag

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans