Human HbA1c (Glycated Hemoglobin A1c) ELISA Kit | ELK5487

Human HbA1c (Glycated Hemoglobin A1c) ELISA Kit | ELK5487 | Gentaur UK, US & Europe Distribution

Alternative Names: Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Species: Human

Assay Type: Sandwich

Sensitivity: 1.36 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: plasma

Assay Lenght: 3.5h

Research Area: Diagnosis of diabetes mellitus

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycated Hemoglobin A1c (HbA1c) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycated Hemoglobin A1c (HbA1c) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycated Hemoglobin A1c (HbA1c), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycated Hemoglobin A1c (HbA1c) in the samples is then determined by comparing the OD of the samples to the standard curve.