855
Human sCTLA4 (Soluble Cytotoxic T-Lymphocyte Associated Antigen 4) ELISA Kit | ELK9343
- SKU:
- 855-ELK9343-GEN
Description
Human sCTLA4 (Soluble Cytotoxic T-Lymphocyte Associated Antigen 4) ELISA Kit | ELK9343 | Gentaur UK, US & Europe Distribution
Alternative Names: CD152; CELIAC3; CTLA4; GSE; IDDM12; Celiac Disease 3; Cytotoxic T-lymphocyte protein 4
Species: Human
Assay Type: Sandwich
Sensitivity: 9.38 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Immune molecule;Autoimmunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (sCTLA4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (sCTLA4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (sCTLA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (sCTLA4) in the samples is then determined by comparing the OD of the samples to the standard curve.