Human sCTLA4 (Soluble Cytotoxic T-Lymphocyte Associated Antigen 4) ELISA Kit | ELK9343

Human sCTLA4 (Soluble Cytotoxic T-Lymphocyte Associated Antigen 4) ELISA Kit | ELK9343 | Gentaur UK, US & Europe Distribution

Alternative Names: CD152; CELIAC3; CTLA4; GSE; IDDM12; Celiac Disease 3; Cytotoxic T-lymphocyte protein 4

Species: Human

Assay Type: Sandwich

Sensitivity: 9.38 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Immune molecule;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (sCTLA4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (sCTLA4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (sCTLA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (sCTLA4) in the samples is then determined by comparing the OD of the samples to the standard curve.