HY5 | Protein long hypocotyl 5 | AS12 1867

HY5 | Protein long hypocotyl 5 | AS12 1867 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide, derived from Arabidopsis thaliana HY5 protein sequence, UniProt:O24646, TAIR: AT5G11260. Chosen peptide is not conserved in HY5 protein sequence.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: Collection of antibodies to proteins involved in photomorphogenesisPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1: 500 to 1 : 1000 (WB)

Molecular weight: 18, 5 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Brassica pekinensisSpecies of your interest not listed? Contact us

Not reactive in: Hordeum vulgare, Oryza sativa, Pisum sativum, Populus sp., Solanum lycopersicum, Triticum aestivum

Additional Information: This antibody detects recombinant HY5 in Nicotiana benthamiana.Extraction and loading buffer with 6-8 M urea buffer needs to be used when working with endogenous extract to allow detection with this antibody or TCA/acetone protein extraction or as described in Mechin et al. (2007) .

Background: HY5 (Protein long hypocotyl 5) is a light-regulated transcription factor that promotes photomorphogenesis in light. Involved in the blue light specific pathway. Heterodimer. Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs. Localized in nucleus. Alternative names: Protein LONG HYPOCOTYL 5, bZIP transcription factor 56, AtbZIP56.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G11260

Category: Photomorphogenesis

Research Area: Plant Biology