Hybrid-R Blood RNA is a complete kit with ready-to-use reagent for the isolation of total RNA from up to 0.25mL whole blood sample. This kit utilizes the lysis method of RiboEx™ LS which has a powerful ability of cell-lysis and the purification method based on glassfiber membrane technology. Fast and convenient procedure of Hybrid-R™ Blood RNA takes only 30 minutes for complete preparation of pure RNA. Whole blood sample is homogenized and lysed in RiboEx™ LS, a monophasic solution containing phenol and guanidium salt, which rapidly lyse cells and inactivates nucleases. In conventional methods, the erythrocytes of mammalian blood which does not contain nuclei (and therefore, RNA either) should be removed by pre-treatment such as osmotic lysis for the separation of leukocytes from whole blood. This additional treatments increase the experiment time and the possibility of RNA breakage, followed by decline of RNA-quality. Hybrid-R Blood RNA Prep does not need the additional treatment of blood sample, and whole blood is lysed in RiboEx™ LS in just one step. Then addition of chloroform brings about a separation of the lysate into aqueous and organic phases. After phase-separating, DNA and protein remains in the interphase and the organic phase respectively but released RNA exists in the aqueous phase. The aqueous phase is picked and applied to a EzPure™ to eliminate small amount of contaminated DNA and other blood contaminants. The passed-through is mixed with buffer RB1, RNA binding buffer, and then the mixture is applied to a mini spin column. After a series of washing with buffer RBW and RNW, pure RNA can be eluted by RNase-free water. Hybrid-R Blood RNA is suitable for RNA preparation from 0.1mL to 0.25mL mammalian whole blood. The typical yield is 3μg per 0.25mL whole blood. The purified RNA can be applicable for the isolation of Poly A+ RNA, Northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RPA and other analytical procedures.

Kit Sizes:

315-150: 50 samples

Applications:

Isolation of total RNA from up to 0.25mL whole blood sample.

Key Features:

• Preparation time: ~30 minutes.
• Accurate and consistent yield from whole blood.
• High purity and yield.
• Sample size: 100 ~250 μL/prep.
• Instant use: No need of additional materials.
• No ethanol precipitation.
• No Genomic DNA contamination.
• Ready for use in northern blotting, dot blotting, in vitro translation, cloning, RT-PCR  and other analytical procedures.

Storage Conditions:

Hybrid-R™ Blood RNA kit except RiboEx™ LS solution should be stored at room temperature. RiboEx™ LS solution should be stored at 4℃ for optimal performance. All components are stable for 1 year.