IFNE (Human) ELISA Kit | E5061-100

IFNE (Human) ELISA Kit | E5061-100

Description: Interferon epsilon (IFNE) is type I interferon which stands out through its unusual expression profile and differing regulation compared to classic type I interferons such as interferon alpha and interferon beta. Type I interferon is required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. It directly mediates protection against viral and bacterial genital infections. It is expressed constitutively in the female reproductive tract, and contributes to protection in models of sexually transmitted infections. ’s IFNE (Human) ELISA Kit is used for the quantitative detection of IFNE in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the lls subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IFNE in the sample.

Alternate Name: IFNE ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit

Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of IFNE

Summary: N/A

Detection Method: Absorbance is measured at 450 nm

Sample Type: Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity: Human

Applications: The ELISA kit is used for the quantitative detection of IFNE in serum, plasma, tissue homogenates and other biological fluids

Features & Benefits: Sensitivity: 9.375 pg/ml Detection range: 15.625-1000 pg/ml Recovery Rate: 96-102% for serum, 92-97% for EDTA plasma and 86-100% for heparin plasma Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Highly sensitive and specific