Description
Immortalized Mouse Enteric Neuronal Cells | T0297
Immortalization Method: Isolation from transgenic mouse with SV-40 large T antigen mutation BioSafety Level: II Organism: H-2Kb-tsA58 Transgenic Mice Species: Mouse Source Organ: Intestine of E13 Mouse Fetus Organ Type: Digestive Growth Properties: Adherent Morphology: Epithelial-like Passage Number: N/A Population Doubling: 25-35 hours Seeding Density: 20,000 - 50,000 cells/cm10²; Recommended split ratio is 1:2 to 1:6 Markers: Peripherin, HuD, PGP9.5, tau, synaptophysin, characteristic enteric neuron receptors, cRet, nestin Donor Age: N/A Donor Gender: N/A Donor Ethnicity: N/A Propagation: The base medium for this cell line is Prigrow IV available from abm . To make the complete growth medium, add the following components to the base medium: 52 ng/ml selenium (Sigma), 16.11 µg/ml putrescine (Sigma), 5 µg/ml insulin (Sigma), 1 µg/ml transferrin (Sigma), 0.1 mg/ml fetuin (Sigma, Cat No. F3385), 1 µg/ml BSA (Bio Basic), 6.3 ng/ml progesterone (Sigma), and L-glutamine to a final concentration of 1%. Quality Control: Neuronal markers were assessed and confirmed via western blot and immunocytochemical analysis. Shipping Condition: Dry Ice Storage Condition: liquid nitrogen or -180C
Filter the media with 0.45 µM filter before proceeding to add fetal bovine serum to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, 10 ng/ml mouse IFN-gamma (Z200085), and 100 ng/ml glial cell line-derived neurotrophic factor (GDNF) (Z101057) to make complete growth media. Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.