Immortalized Mouse Enteric Neuronal Cells | T0297

Immortalized Mouse Enteric Neuronal Cells | T0297

Immortalization Method:

Isolation from transgenic mouse with SV-40 large T antigen mutation

BioSafety Level:

II

Organism:

H-2Kb-tsA58 Transgenic Mice

Species:

Mouse

Source Organ:

Intestine of E13 Mouse Fetus

Organ Type:

Digestive

Growth Properties:

Adherent

Morphology:

Epithelial-like

Passage Number:

N/A

Population Doubling:

25-35 hours

Seeding Density:

20,000 - 50,000 cells/cm10²; Recommended split ratio is 1:2 to 1:6

Markers:

Peripherin, HuD, PGP9.5, tau, synaptophysin, characteristic enteric neuron receptors, cRet, nestin

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV available from abm . To make the complete growth medium, add the following components to the base medium: 52 ng/ml selenium (Sigma), 16.11 µg/ml putrescine (Sigma), 5 µg/ml insulin (Sigma), 1 µg/ml transferrin (Sigma), 0.1 mg/ml fetuin (Sigma, Cat No. F3385), 1 µg/ml BSA (Bio Basic), 6.3 ng/ml progesterone (Sigma), and L-glutamine to a final concentration of 1%.

Filter the media with 0.45 µM filter before proceeding to add fetal bovine serum to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, 10 ng/ml mouse IFN-gamma (Z200085), and 100 ng/ml glial cell line-derived neurotrophic factor (GDNF) (Z101057) to make complete growth media.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Neuronal markers were assessed and confirmed via western blot and immunocytochemical analysis.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C