Iron assay kit (Ferrozine)

Iron assay kit (Ferrozine)

Product Description

Iron is a mineral which binds many proteins and enzymes, and plays important roles in living bodies. MC-Reagent Fe Assay Kit provides a simple and convenient tool for the determination of Ferrous and/or Ferric ion in a variety of biological samples such as cell lysates, tissue homogenates, serum, plasma, saliva, urine, tissue extract, plant extract, hair and food extract.

Specifications

Assay principle: Ferrozine method (Colorimetric assay at 560nm) Specifity: Specific to Fe2+ and Fe3+ Measuring range: 5 - 1,000 µg/dL Sample volume: 40 µL Assay conditions: 10 minutes at room temperature (25 - 37 °C) Applications: Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Metal chelatos, for example EDTA may interfere with this assay. Storage temperature: 2 - 8°C (don't freeze). Expiry date: 1 year after the day of manufacturing. Required but not provided: 96-well plate Pipeting devices such as 8-channel (50-200 µL) micropipettor and tips 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. Microtiter plate reader (measuring wavelength between 540 and 580nm. 560nm is recommended.)

Content of this kit

R-A Buffer (ready to use): 1 x 40 mL R-R Chelate color (ready to use): 1 x1.6 mL Iron standard 200 µg/dL (ready to use): 1 x 8 mL

References: Assay examples

1) Y Ikeda, S Tajima, YI-Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya and T Tamaki: Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes. PLoS ONE 7(7),e40465(2012) doi: 10.1371/journal.pone.0040465 2) Tsugawa H, Suzuki H, Matsuzaki J, Hirata K, Hibi T: FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach. Free Radical Biology and Medicine 52(6),p1003-1010(2012) doi: 10.1016/j.freeradbiomed.2011.12.011. 3) M Nakaya, M Tajima, H Kosako, T Nakaya, A Hashimoto, K Watari, H Nishihara, M Ohba, S Komiya, N Tani, M Nishida, H Taniguchi, Y Sato, M Matsumoto, M Tsuda, M Kuroda, K Inoue and H Kurose: GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance. Nature Communications 4, 1532 (2013) doi:10.1038/ncomms2540 4) Y Ikeda, H Enomoto, S Tajima, YI Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya, T Tamaki: Dietary iron restriction inhibits progression of diabetic nephropathy in db/db mice. American Journal of Physiology - RenalPhysiology 304:F1028-F1036(2013) DOI:10.1152/ajprenal.00473.2012 5) Y Ikeda, I Ozono, S Tajima, M Imao, Y Horinouchi, YI Ishizawa, Y Kihira, L Miyamoto, K Ishizawa, K Tsuchiya, T Tamaki: Iron Chelation by Deferoxamine Prevents Renal Interstitial Fibrosis in Mice with Unilateral Ureteral Obstruction. PLoS ONE 9(2), e89355(2014) doi:10.1371/journal.pone.0089355